Difference between revisions of "Part:BBa K2448016:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
pPsiTac1
 
−
 
  
 +
pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the binding sequence of a transcription factor, PsiR (BBa_K2448006).
  
 
===Source===
 
===Source===
  
−
pPsiTac1
+
DNA synthesis
  
 
===References===
 
===References===
 +
 +
[1] de Boer et al. The tac promoter: A functional hybrid derived from the trp and lac promotersPNAS (1983) 80, 21-25.
 +
[2] http://regprecise.lbl.gov/RegPrecise/sites.jsp?regulog_id=5087

Revision as of 11:05, 28 October 2017


pPsiTac1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the binding sequence of a transcription factor, PsiR (BBa_K2448006).

Source

DNA synthesis

References

[1] de Boer et al. The tac promoter: A functional hybrid derived from the trp and lac promotersPNAS (1983) 80, 21-25. [2] http://regprecise.lbl.gov/RegPrecise/sites.jsp?regulog_id=5087