Difference between revisions of "Part:BBa K2455000:Design"

 
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===Source===
 
===Source===
  
−
The sequence originated from the MG1655 E. coli strain but carries a Pro150Leu point mutation in order to avoid negative feedback inhibition by L-phenylalanine (source - Gu et al has further link), in addition a hexa his-tag has been added.
+
The sequence originated from the MG1655 E. coli strain but carries a Pro150Leu point mutation in order to avoid negative feedback inhibition by L-phenylalanine ([https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-11-30 Gu <i>et al.</i>, 2012]), in addition a hexa his-tag has been added.
  
 
===References===
 
===References===

Revision as of 10:57, 28 October 2017


His-tagged AroG gene from E. coli MG1655


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 241


Design Notes

In addition to having a P150L mutation the AroG sequence contains a his-tag in order to allow for detection of expression through the use of Western Blotting.


Source

The sequence originated from the MG1655 E. coli strain but carries a Pro150Leu point mutation in order to avoid negative feedback inhibition by L-phenylalanine (Gu et al., 2012), in addition a hexa his-tag has been added.

References