Difference between revisions of "Part:BBa K2455000"
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AroG is the first enzyme of the shikimate pathway leading to synthesis of chorismate, which subsequently can be converted to tryptophan, tyrosine, and phenylalanine. Being the first enzyme in the pathway, it determines the carbon flow towards amino acid synthesis and thus the production. | AroG is the first enzyme of the shikimate pathway leading to synthesis of chorismate, which subsequently can be converted to tryptophan, tyrosine, and phenylalanine. Being the first enzyme in the pathway, it determines the carbon flow towards amino acid synthesis and thus the production. | ||
| − | Previous work ([https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-11-30 Gu <i>et al. | + | Previous work ([https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-11-30 Gu <i>et al.</i>, 2012]) have shown that overexpression of AroG leads to increased tryptophan production in E. coli. |
Latest revision as of 10:54, 28 October 2017
His-tagged AroG gene from E. coli MG1655
AroG gene from E. coli strain MG1655 carrying an P150L point mutation (to avoid negative feedback inhibition) and a hexa histidine-tag attached by a short linker sequence.
AroG is the first enzyme of the shikimate pathway leading to synthesis of chorismate, which subsequently can be converted to tryptophan, tyrosine, and phenylalanine. Being the first enzyme in the pathway, it determines the carbon flow towards amino acid synthesis and thus the production.
Previous work (Gu et al., 2012) have shown that overexpression of AroG leads to increased tryptophan production in E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 934
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 241