Difference between revisions of "Part:BBa K2235011"
(→‎Usage and Biology)
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==Usage and Biology==
 
==Usage and Biology==
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We used an already existing biobrick for HylA E.coli secretion system (BBa_K1166002) to secrete our sialidase from the iGEM 2017 distribution kit.
  
 
==Characterization==
 
==Characterization==

Revision as of 10:37, 28 October 2017


Sialidase enzyme coding composite N terminally attached to secretion system type 1

Usage and Biology

We used an already existing biobrick for HylA E.coli secretion system (BBa_K1166002) to secrete our sialidase from the iGEM 2017 distribution kit.

Characterization

Important Parameter

Purification and identification

Ligation of sialidase insert into secretion device

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3178
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3117
    Illegal XhoI site found at 127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 739
    Illegal AgeI site found at 2952
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1119
    Illegal SapI site found at 2934