Difference between revisions of "Part:BBa K2235009:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | The following primers were used to amplify the gblock: | ||
+ | Fwd: TATATGAATTCGCGGCCGCTTCTAGAGTAATACG | ||
+ | Rev: ATATTCTGCAGCGGCCGCTACTAGTATTAGATGG | ||
+ | |||
His tag is N-terminally attached to the Sialidase enzyme coding sequence. | His tag is N-terminally attached to the Sialidase enzyme coding sequence. | ||
Revision as of 08:29, 28 October 2017
Sialidase composite with T7 promoter and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 127
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 574
Illegal NgoMIV site found at 649
Illegal NgoMIV site found at 739 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1119
Design Notes
The following primers were used to amplify the gblock: Fwd: TATATGAATTCGCGGCCGCTTCTAGAGTAATACG Rev: ATATTCTGCAGCGGCCGCTACTAGTATTAGATGG
His tag is N-terminally attached to the Sialidase enzyme coding sequence.
Source
References
Christensen, S. and Egebjerg, J. (2005), Cloning, expression and characterization of a sialidase gene from Arthrobacter ureafaciens. Biotechnology and Applied Biochemistry, 41: 225–231. doi:10.1042/BA20040144