Difference between revisions of "Part:BBa K2244006"

Latest revision as of 08:22, 28 October 2017

ColE promoter

ColE promoter is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.

Usage and Biology

ColE promoter is 151bp in length. We used a light-repressed system containing a single light repressor LexA-VVD, and a ColE promoter that contains a SOS operator sequence for LexA binding to regulate the expression of target genes. The picture shows a colony PCR product of ColE promoter in pLEV1(408) vector.

Reference

Tomizawa, J. 1985. Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible.Cell. 40(3):527-35.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 08:17, 28 October 2017 (view source) YuhangYang (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Latest revision as of 08:22, 28 October 2017 (view source) YuhangYang (Talk \| contribs)
Line 4:		Line 4:


−	~~Co1E~~ promoter is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.	+	ColE promoter is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.