Difference between revisions of "Part:BBa K2486114"
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<partinfo>BBa_K2486114 short</partinfo> | <partinfo>BBa_K2486114 short</partinfo> | ||
| − | . | + | [theoretical part] [not assembled, not synthesized, not sent, not characterized] |
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| + | Anti malarial peptide derived from ''Apis mellifera'' phospholipase A2 (1). Modified to not hold enzymatic activities (2) but still be effective. Used in paratransgenic approaches to block ''Plasmodium'' transmission ''in vivo''. (3) | ||
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| + | GenBank: https://www.ncbi.nlm.nih.gov/nuccore/X16709 | ||
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| + | 1- Moreira, Luciano A., et al. "Bee venom phospholipase inhibits malaria parasite development in transgenic mosquitoes." Journal of Biological Chemistry 277.43 (2002): 40839-40843. | ||
| + | |||
| + | 2 - Rodrigues, F. G., et al. "Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development." Insect molecular biology 17.2 (2008): 175-183. | ||
| + | |||
| + | 3 - Wang, Sibao, et al. "Driving mosquito refractoriness to Plasmodium falciparum with engineered symbiotic bacteria." Science 357.6358 (2017): 1399-1402. | ||
| + | |||
| + | Sequence codon optized to E. coli with https://www.idtdna.com/CodonOpt | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 04:47, 28 October 2017
Anti malarial peptide mPLA2 (no stop codon)
[theoretical part] [not assembled, not synthesized, not sent, not characterized]
Anti malarial peptide derived from Apis mellifera phospholipase A2 (1). Modified to not hold enzymatic activities (2) but still be effective. Used in paratransgenic approaches to block Plasmodium transmission in vivo. (3)
GenBank: https://www.ncbi.nlm.nih.gov/nuccore/X16709
1- Moreira, Luciano A., et al. "Bee venom phospholipase inhibits malaria parasite development in transgenic mosquitoes." Journal of Biological Chemistry 277.43 (2002): 40839-40843.
2 - Rodrigues, F. G., et al. "Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development." Insect molecular biology 17.2 (2008): 175-183.
3 - Wang, Sibao, et al. "Driving mosquito refractoriness to Plasmodium falciparum with engineered symbiotic bacteria." Science 357.6358 (2017): 1399-1402.
Sequence codon optized to E. coli with https://www.idtdna.com/CodonOpt
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]