Difference between revisions of "Part:BBa K2486004"

Revision as of 04:35, 28 October 2017

dtxR generator

The BBa_K486004 is a dtxR constitutive generator. This part is composed of:

A promoter (BBa_J23108) from Anderson promoter collection.
A ribosome binding site BBa_B0034
A short spacer of 6bp (gtccta)
dtxR gene BBa K2486005
A short spacer of 16bp (ccagctcagtccgcat)
A synthetic terminator (BBa_K2486006)

REFERENCES:

Boyd, J., Oza, M. N., & Murphy, J. R. (1990). Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. Proceedings of the National Academy of Sciences, 87(15), 5968-5972.

De Zoysa, A., Efstratiou, A., & Hawkey, P. M. (2005). Molecular characterization of diphtheria toxin repressor (dtxR) genes present in nontoxigenic Corynebacterium diphtheriae strains isolated in the United Kingdom. Journal of clinical microbiology, 43(1), 223-228.

https://www.wikigenes.org/e/gene/e/2649688.html

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 509
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 The BBa_K486004 is a dtxR constitutive generator. This part is composed of:
-# A promoter ([https://parts.igem.org/wiki/index.php/Part:BBa_J23108 BBa_J23108]) from [https://parts.igem.org/Promoters/Catalog/Anderson Anderson promoter collection]
+# A promoter ([https://parts.igem.org/wiki/index.php/Part:BBa_J23108 BBa_J23108]) from [https://parts.igem.org/Promoters/Catalog/Anderson Anderson promoter collection].
-# A ribosome binding site
+# A ribosome binding site [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
 # A short spacer of 6bp (gtccta)
-# dtxR gene
+# dtxR gene [https://parts.igem.org/Part:BBa_K2486005 BBa K2486005]
 # A short spacer of 16bp (ccagctcagtccgcat)
-#
+# A synthetic terminator (BBa_K2486006)