Latest revision as of 04:11, 28 October 2017

hydrogen sensor sequence, HoxA-HoxB-HoxC-HoxJ-terminator-HoxP-EGFP

A composite of hydrogen sensor full length sequence. The order of the elements is: HoxA-HoxB-HoxC-HoxJ-terminator-HoxP-EGFP. The sequence of HoxABCJP comes from Ralstonia eutropha H16 megaplasmid pHG1. The hole sequence acts as an hydrogen sensor.

Usage and Biology

Design

This design is Inspired from Cupriavidusnecator H16.

Experiments

Now we’ve succefully detected the protein expression by SDS-Page analysis and Western blot analysis.

Figure 1. Coomassie Brilliant Blue R-250-stained SDS-Page analysis of recombinant E.coli expressing hoxABCJ-terminator-hoxp-gfp

Fingure 2. Western blot analysis of recombinant E.coli expressing his-hoxA

Fluorescence intensity remains stationary when IPTG is added.
And Fluorescence intensity increases in a low hydrogen atmosphere.
When the amount of hydrogen goes to a higher level Fluorescence intensity increases apparently. meaning the designed reporter pathway have worked.

Figure 3. Influence of H2 concentration on fluorescence expression

File:T-Nanjing-China-h2-Data 1.tif

Sequence and Features