Difference between revisions of "Part:BBa I746220"

 
 
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<partinfo>BBa_I746220 short</partinfo>
 
<partinfo>BBa_I746220 short</partinfo>
  
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This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.
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In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick detects group I AIP.
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The device incorporates the sensing infrastructure described [https://parts.igem.org/wiki/index.php/Part:BBa_I746101 here] with the permeability device [https://parts.igem.org/wiki/index.php/Part:BBa_I746201 here] so that the signal peptide is able to cross the outer membrane of E. coli to be detected.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:50, 24 October 2007

AIP Detector

This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.

In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick detects group I AIP.

The device incorporates the sensing infrastructure described here with the permeability device here so that the signal peptide is able to cross the outer membrane of E. coli to be detected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3105
    Illegal NheI site found at 3128
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 426
    Illegal BamHI site found at 1310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3397
    Illegal AgeI site found at 3763
    Illegal AgeI site found at 4390
    Illegal AgeI site found at 4908
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2877