Difference between revisions of "Part:BBa K2289000:Design"

 
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===References===
 
===References===
 +
X. Zheng, B. Hu, SX. Gao, D.J. Liu, M.J. Sun, B.H. Jiao, L.H. Wang. (2015). A saxitoxin-binding aptamer whith higher affinity and inhibitory activity optimized by rational site-directed mutagenesis and truncation. Toxicon 101. 41-47.

Revision as of 03:53, 28 October 2017


STX Aptazyme J_1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a noncoding ssDNA sequence, for use you have to order directly to IDT.


Source

The DNAzyme sequence is on the Registry Part:BBa_K1614007 and it was submited by team Heidelberg 2015. Aptamer sequence, the STX M30f, was selected by a pool of aptarmers described in Zheng et. al., 2015. And the linkers where selected by the JAWS software developed by team Heidelberg, for this special case we selected de J_1 linkers.

References

X. Zheng, B. Hu, SX. Gao, D.J. Liu, M.J. Sun, B.H. Jiao, L.H. Wang. (2015). A saxitoxin-binding aptamer whith higher affinity and inhibitory activity optimized by rational site-directed mutagenesis and truncation. Toxicon 101. 41-47.