Difference between revisions of "Part:BBa K2325102"
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| + | Using the pGEX-2T vector, the PTDH could be expressed in BL21(DE3). | ||
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[[File:BBa_K2325102--1.png|750px]]<br /> | [[File:BBa_K2325102--1.png|750px]]<br /> | ||
Revision as of 03:30, 28 October 2017
Phosphite dehydrogenase expression device
The BBa_K2325101 contains formamidase gene with T7 promoter and terminator.
Usage and Biology
Using the pGEX-2T vector, the PTDH could be expressed in BL21(DE3).
Figure 1. Growth curve of E.coli BL21(DE3) and the engineering E.coli BL21(DE3)(pGEX-ptx) expressing phosphite dehydrogenase in a medium contains Na2HPO3•5H20 (1.32mM) and NH4Cl serving as the phosphorus and nitrogen sources, respectively.
The E.coli expressing phosphite dehydrogenase outcompeted a control strain in phosphite-containing media after 72 hours. However, the negative control group still grew in a certain degree. We looked up the researches and presumed that, alkaline phosphatase(BAP) found in E.coli, will be increasingly express when the amount of bio-available phosphorus is low. Through the hydrolysis, the BAP then turn the phosphorus compounds in the environment into bio-available phosphorus. With the help of the BAP, the microbes can utilize the phosphorus compounds more effectively, which also facilitates the modified pathways.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]