Difference between revisions of "Part:BBa K516031"

Revision as of 03:05, 28 October 2017

mRFP protein generator TERM+ RBS+(B0031)

Red fluorescent protein generator with BBa_B0031 low efficiency RBS (from Community Collection) and double terminator.
This construct is useful to measure the relative strength of promoters or the RBS efficiency in relationship with a promoter of interest.
To build a measurement device is only necessary assemble a standard biobrick promoter with this device (or another one of the collection: BBa_K516030 ; BBa_K516032)

Contribution from uOttawa iGEM 2017
Author: Setti Belhouari
Biobrick BBa_B0031 has been transformed into E. coli strain Mach1. Though five colonies have grown overnight, all of them tested negative for the emission of RFP through flow cytometry when compared to a zero control Mach1 strain. Perhaps expression expression of RFP may be induced using a stronger promoter.
Picture of plate.
CSV files.
Histograms.

Sequence and Features