Difference between revisions of "Part:BBa K2325002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We cloned FOR and PTDH genes into the plasmid pGEX-2T, and got the recombinant plasmid pGEX-for-ptx. | + | We cloned FOR and PTDH genes into the plasmid pGEX-2T, and got the recombinant plasmid pGEX-for-ptx where FOR and PTDH form a fusion protein. |
===Gel analysis=== | ===Gel analysis=== | ||
Latest revision as of 02:36, 28 October 2017
Formamidase & Phosphite dehydrogenase fusion protein
It is a fusion protein of formamidase and phosphite dehydrogenase with a linker between them.
Usage and Biology
We cloned FOR and PTDH genes into the plasmid pGEX-2T, and got the recombinant plasmid pGEX-for-ptx where FOR and PTDH form a fusion protein.
Gel analysis
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 69
Illegal NgoMIV site found at 556
Illegal NgoMIV site found at 751
Illegal NgoMIV site found at 898 - 1000COMPATIBLE WITH RFC[1000]