Difference between revisions of "Part:BBa K2232028"

Latest revision as of 02:12, 28 October 2017

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Sequence and Features

Assembly Compatibility:

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 <partinfo>BBa_K2232028 short</partinfo>
-This part upregulates an operon responsible for germination rate. Bacillus subtilis spores germinate in the presence of L-Alanine. Up-regulation of the spoVA operon increases germination rate in response to L-Alanine. The promoter for the B.subtilis sspB gene (PsspB) is more active than the endogenous spoVA promoter. It is also active during germination. Expression of the spoVA operon under PsspB increases the germination rate.
-This construct consists mainly of the sspB promoter followed by the first 354bp of the spoVA operon (first 354bp of the spoVAA gene). Since B.subtilis exhibits accurate and efficient homologous recombination, a single cross-over event between the spoVAA region in the BioBrick and the endogenous spoVAA sequence inserts the sspB promoter upstream of the spoVA operon.
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