Difference between revisions of "Part:BBa K2302011"
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This plasmid cords for fatty acid binding protein and anchor it on the outer membrane, it consists four basic parts: promoter T7, RBS, Lpp-OmpA, and L-FABP. Lpp-OmpA is sequence cords for a functional peptide, which give efficient surface anchoring of FABP, and FABP is a kind of protein which can bind long chain fatty acid. By transforming this plasmid, a bacteria can catch long chain fatty acid in liquid and carry it on its membrane.
 
This plasmid cords for fatty acid binding protein and anchor it on the outer membrane, it consists four basic parts: promoter T7, RBS, Lpp-OmpA, and L-FABP. Lpp-OmpA is sequence cords for a functional peptide, which give efficient surface anchoring of FABP, and FABP is a kind of protein which can bind long chain fatty acid. By transforming this plasmid, a bacteria can catch long chain fatty acid in liquid and carry it on its membrane.
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===Usage and Biology===
 
  
 
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Revision as of 01:22, 28 October 2017


T7-His-Lpp-ompA-L-FABP-T7terminater

This plasmid cords for fatty acid binding protein and anchor it on the outer membrane, it consists four basic parts: promoter T7, RBS, Lpp-OmpA, and L-FABP. Lpp-OmpA is sequence cords for a functional peptide, which give efficient surface anchoring of FABP, and FABP is a kind of protein which can bind long chain fatty acid. By transforming this plasmid, a bacteria can catch long chain fatty acid in liquid and carry it on its membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 491
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716