Difference between revisions of "Part:BBa K2447013"
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TlpA36 protein is constitutively produced, and depending on the temperature it will either form dimers capable of repressing the pTlpA36 promoter (in temperatures below 36 degrees) or stay in monomeric form which is unable to repress the promoter. | TlpA36 protein is constitutively produced, and depending on the temperature it will either form dimers capable of repressing the pTlpA36 promoter (in temperatures below 36 degrees) or stay in monomeric form which is unable to repress the promoter. | ||
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| + | As a proof of concept that the temperature system is fully functional, we had constructed and characterised an [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2447014 ITPG-inducible temperature system] and coupled it to a downstream GFP reporter. | ||
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Revision as of 00:47, 28 October 2017
Temperature-sensitive repressible system
TlpA36 protein is constitutively produced, and depending on the temperature it will either form dimers capable of repressing the pTlpA36 promoter (in temperatures below 36 degrees) or stay in monomeric form which is unable to repress the promoter.
As a proof of concept that the temperature system is fully functional, we had constructed and characterised an ITPG-inducible temperature system and coupled it to a downstream GFP reporter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 526
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 811
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1114
- 1000COMPATIBLE WITH RFC[1000]