Difference between revisions of "Part:BBa K876070:Experience"
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===Applications of BBa_K876070=== | ===Applications of BBa_K876070=== | ||
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| + | '''Group:'''iGEM_BIT2017 | ||
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| + | '''Author:'''WENJIA WANG | ||
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| + | We transformed the part BBa_K876070 into the E.coli ΔLysA, and cultured in LB medium overnight. Then aliquots of 100ul were transferred into M9 medium to deplete lysine. After 10h, aliquots of 100ul were transferred into M9 medium with lysine. Lysine was added in increments of 0.5ug/mL to establish a range of 0-3ug/mL. OD600 was measured with microplate reader every hour. The result shows that the OD600 and GFP fluorescence grows obviously with the concentration gradient of the lysine. But its growth rate and GFP fluorescence is much lower than that in LB medium. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 00:19, 28 October 2017
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Applications of BBa_K876070
Group:iGEM_BIT2017
Author:WENJIA WANG
We transformed the part BBa_K876070 into the E.coli ΔLysA, and cultured in LB medium overnight. Then aliquots of 100ul were transferred into M9 medium to deplete lysine. After 10h, aliquots of 100ul were transferred into M9 medium with lysine. Lysine was added in increments of 0.5ug/mL to establish a range of 0-3ug/mL. OD600 was measured with microplate reader every hour. The result shows that the OD600 and GFP fluorescence grows obviously with the concentration gradient of the lysine. But its growth rate and GFP fluorescence is much lower than that in LB medium.
User Reviews
UNIQ6655b0eef10eac89-partinfo-00000000-QINU UNIQ6655b0eef10eac89-partinfo-00000001-QINU