Difference between revisions of "Part:BBa K2305013"
 
Line 7: Line 7:
 
We calculated the new part’s base number to be 2389bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
 
We calculated the new part’s base number to be 2389bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
 
[[Image:BIT Figure DNA sequencing K2305013.png|200px|left|K230500]]<br clear="all">
 
[[Image:BIT Figure DNA sequencing K2305013.png|200px|left|K230500]]<br clear="all">
 +
==Characterization map==
 +
[[Image:BIT Figure map K2305013.png|400px|left|K230500]]<br clear="all">
 +
Fig.The Growth curve of E.coil.
 +
  
  

Latest revision as of 22:46, 27 October 2017


pbad RBS Lacl

promoter pbad and LacI generator with RBS

Agarose gel electrophoresis

We calculated the new part’s base number to be 2389bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230500

Characterization map

K230500

Fig.The Growth curve of E.coil.


DNA sequencing

The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

K230500
K230500


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2383
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961