Difference between revisions of "Part:BBa K2267040"

 
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This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.
 
This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.
 +
<!-- Add more about the biology of this part here
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===Usage and Biology===
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Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
 
Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
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In this case, the constitutively expressed LuxR transcriptional regulator (C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device. We are also currently characterising the cross-talk of the Lux receiver device with other quorum-sensing systems, in order to determine its orthogonality.
+
In this case, the constitutively expressed LuxR transcriptional regulator (C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.
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Sequence and Features
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我们以    为Control，
 +
part1: https://parts.igem.org/Part:BBa_K2267026+https://parts.igem.org/Part:BBa_K2267029+https://parts.igem.org/Part:BBa_K319039.
  
 +
part3: https://parts.igem.org/Part:BBa_K2267026+ https://parts.igem.org/Part:BBa_K2267030+https://parts.igem.org/Part:BBa_K319039.
  
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<!-- Add more about the biology of this part here
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part4: https://parts.igem.org/Part:BBa_K2267026+ https://parts.igem.org/Part:BBa_K2267031+https://parts.igem.org/Part:BBa_K319039.
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===Usage and Biology===
+
 
 +
part6: https://parts.igem.org/Part:BBa_K2267026+ https://parts.igem.org/Part:BBa_K2267032+https://parts.igem.org/Part:BBa_K319039.
 +
 
 +
part8: https://parts.igem.org/Part:BBa_K2267026+ https://parts.igem.org/Part:BBa_K2267033+https://parts.igem.org/Part:BBa_K319039.
 +
 
 +
part1:  https://parts.igem.org/Part:BBa_K2267043 </br>
 +
 
 +
part3:  https://parts.igem.org/Part:BBa_K2267041 </br>
 +
 
 +
part4:  https://parts.igem.org/Part:BBa_K2267042 </br>
 +
 
 +
part6:  https://parts.igem.org/Part:BBa_K2267044 </br>
 +
 
 +
part8:  https://parts.igem.org/Part:BBa_K2267045 </br>
 +
 
 +
===result===
  
 +
https://static.igem.org/mediawiki/2017/e/e6/T--TUST_China--part_part_results.png
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 22:26, 27 October 2017


LuxR-I-Plux-GFP

This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. In this case, the constitutively expressed LuxR transcriptional regulator (C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device. 我们以 为Control， part1: https://parts.igem.org/Part:BBa_K2267026+https://parts.igem.org/Part:BBa_K2267029+https://parts.igem.org/Part:BBa_K319039.

part3: https://parts.igem.org/Part:BBa_K2267026+ https://parts.igem.org/Part:BBa_K2267030+https://parts.igem.org/Part:BBa_K319039.

part4: https://parts.igem.org/Part:BBa_K2267026+ https://parts.igem.org/Part:BBa_K2267031+https://parts.igem.org/Part:BBa_K319039.

part6: https://parts.igem.org/Part:BBa_K2267026+ https://parts.igem.org/Part:BBa_K2267032+https://parts.igem.org/Part:BBa_K319039.

part8: https://parts.igem.org/Part:BBa_K2267026+ https://parts.igem.org/Part:BBa_K2267033+https://parts.igem.org/Part:BBa_K319039.

part1: https://parts.igem.org/Part:BBa_K2267043 </br>

part3: https://parts.igem.org/Part:BBa_K2267041 </br>

part4: https://parts.igem.org/Part:BBa_K2267042 </br>

part6: https://parts.igem.org/Part:BBa_K2267044 </br>

part8: https://parts.igem.org/Part:BBa_K2267045 </br>

result

T--TUST_China--part_part_results.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 837
    Illegal NheI site found at 860
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 928
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1452
    Illegal BsaI.rc site found at 2179