Difference between revisions of "Part:BBa K2305016"
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<partinfo>BBa_K2305016 short</partinfo>
 
<partinfo>BBa_K2305016 short</partinfo>
  
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luxl generator produce AHL, and AHL combined with luxR products will promote the expression of plux
+
Plux promoter stars the circuit, and Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux and form a cyclic amplifier to achieve a higher GFP expression.
 
==Agarose gel electrophoresis==
 
==Agarose gel electrophoresis==
 
We calculated the new part’s base number to be 2417bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
 
We calculated the new part’s base number to be 2417bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

Revision as of 20:14, 27 October 2017


GFP with cycle amplification system

Plux promoter stars the circuit, and Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux and form a cyclic amplifier to achieve a higher GFP expression.

Agarose gel electrophoresis

We calculated the new part’s base number to be 2417bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230500


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2204