Difference between revisions of "Part:BBa K2305013"
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promoter pbad and LacI generator with RBS | promoter pbad and LacI generator with RBS | ||
| + | ==Agarose gel electrophoresis== | ||
| + | We calculated the new part’s base number to be 2389bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct. | ||
| + | [[Image:BIT Figure DNA sequencing K2305013.png|200px|left|K230500]]<br clear="all"> | ||
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| + | ==DNA sequencing== | ||
| + | The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further | ||
| + | [[Image:BIT Figure DNA sequencing K2305013a.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305013b.png|200px|left|K230500]]<br clear="all"> | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 19:19, 27 October 2017
pbad RBS Lacl
promoter pbad and LacI generator with RBS
Agarose gel electrophoresis
We calculated the new part’s base number to be 2389bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
DNA sequencing
The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2383
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961