Difference between revisions of "Part:BBa K2305010"
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It's a GFP/RFP dual fluorescent system | It's a GFP/RFP dual fluorescent system | ||
| + | ==Agarose gel electrophoresis== | ||
| + | We calculated the new part’s base number to be 1007bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct. | ||
| + | [[Image:BIT Figure DNA sequencing K2305010.png|200px|left|K230500]]<br clear="all"> | ||
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| + | DNA sequencing[edit] | ||
| + | The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further | ||
| + | [[Image:BIT Figure DNA sequencing K2305010a.png|200px|left|K230500]] | ||
| + | [[Image:BIT Figure DNA sequencing K2305010b.png|200px|left|K230500]] | ||
| + | <br clear="all"> | ||
| + | |||
Revision as of 18:56, 27 October 2017
GFP/RFP dual fluorescent system with LacI and plac
It's a GFP/RFP dual fluorescent system
Agarose gel electrophoresis
We calculated the new part’s base number to be 1007bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
DNA sequencing[edit] The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1165
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2846
Illegal AgeI site found at 2958 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1844