Difference between revisions of "Part:BBa K2382005"
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<partinfo>BBa_K2382005 parameters</partinfo>
 
<partinfo>BBa_K2382005 parameters</partinfo>
 
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===Design Notes===
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This is a fusion protein made out of Thioredoxin and FGD.
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Both of them have different function.
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Thioredoxin can help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.
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FGD could help the electron transferred from G6P(Glucose 6-phosphate) to the F420(It is a flavin derivative, and it plays like a coenzyme that could help the redox reactions in methanogens ,many Actinobacteria, especially in bacterial lineages.)
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Finally, the F420 would be reduced to F420H2,attaching two hydrogens and electron on it.
 +
This fusion protein will help us to convert electrons to reduce the compound via MSMEG5998.

Revision as of 18:43, 27 October 2017


Thioredoxin-FGD fusion protein

By ligating these two different parts as a fusion protein, it is supposed to raise the solubility of our protein, F420-dependent glucose-6- phosphate dehydrogenase (FGD).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1117
    Illegal BamHI site found at 405
    Illegal XhoI site found at 411
    Illegal XhoI site found at 1383
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 777
    Illegal NgoMIV site found at 975
    Illegal AgeI site found at 741
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a fusion protein made out of Thioredoxin and FGD. Both of them have different function. Thioredoxin can help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active. FGD could help the electron transferred from G6P(Glucose 6-phosphate) to the F420(It is a flavin derivative, and it plays like a coenzyme that could help the redox reactions in methanogens ,many Actinobacteria, especially in bacterial lineages.) Finally, the F420 would be reduced to F420H2,attaching two hydrogens and electron on it. This fusion protein will help us to convert electrons to reduce the compound via MSMEG5998.