Difference between revisions of "Part:BBa K2267029:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.
  
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NAME SITE HOW before after
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plux1 -8 "delete
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" C
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plux2 -7 "
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delete " G
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plux3 -10 substitute   G A
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plux4 -10 substitute   G T
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plux5 -11 substitute A T
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plux6 -12 substitute T C
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plux7 -12&-11 substitute TA CT
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plux8 -12&-10 substitute TAG CAA
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plux9 -11&-10 substitute AG TT
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plux10 -11&-10 substitute AG TA
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plux11 -12&-10 substitute TAG CAT
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plux12 -12&-11&-10 substitute TAG CTT
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plux13 -12&-11&-9 substitute TAG CTA
 
===Applications of BBa_K2267029===
 
===Applications of BBa_K2267029===
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Promoter activated by LuxR in concert with HSL
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A quorum-sensing system involving LuxR, the transcriptional activator, and an acyl-homserine lactone signal regulate the lux operon in vibrio fischeri.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 18:27, 27 October 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out. This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.


NAME SITE HOW before after plux1 -8 "delete " C plux2 -7 " delete " G plux3 -10 substitute G A plux4 -10 substitute G T plux5 -11 substitute A T plux6 -12 substitute T C plux7 -12&-11 substitute TA CT plux8 -12&-10 substitute TAG CAA plux9 -11&-10 substitute AG TT plux10 -11&-10 substitute AG TA plux11 -12&-10 substitute TAG CAT plux12 -12&-11&-10 substitute TAG CTT plux13 -12&-11&-9 substitute TAG CTA

Applications of BBa_K2267029

Promoter activated by LuxR in concert with HSL A quorum-sensing system involving LuxR, the transcriptional activator, and an acyl-homserine lactone signal regulate the lux operon in vibrio fischeri.

User Reviews

UNIQ1526f659e986ce87-partinfo-00000000-QINU UNIQ1526f659e986ce87-partinfo-00000001-QINU