Difference between revisions of "Part:BBa K2305003"
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==DNA sequencing== | ==DNA sequencing== | ||
The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further | The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further | ||
| − | [[Image:BIT Figure DNA sequencing | + | [[Image:BIT Figure DNA sequencing K2305003a.png|200px|left|K230500]] |
[[Image:BIT Figure DNA sequencing K2305003b.png|200px|left|K230500]]<br clear="all"> | [[Image:BIT Figure DNA sequencing K2305003b.png|200px|left|K230500]]<br clear="all"> | ||
Revision as of 18:11, 27 October 2017
pbad promoter with GFP and lacI
This is a pbad promoter expressing LacI and GFP
Agarose gel electrophoresis
We calculated the new part’s base number to be 2195bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
DNA sequencing
The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1303
Illegal BamHI site found at 65 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1982