Difference between revisions of "Part:BBa K2377009"

 
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===Usage and Biology===
 
===Usage and Biology===
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Improved target:
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BBa_K1755301（K1755024、K1755305、K1755303）
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Improved form
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We replaced a sensor element（Copper-Responsive Two-Component System BBa_K190017）for the Zinc-Responsive Two-Component regulatory system (contains σ54 promoter).
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Mechanism of changed sensor
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The mechanism of Zinc sensor is related to a intrinsic signaling pathway in Ecoli.
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Which is known that many sensing proteins ZraS immobilized on the membrane of cell.
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And when Zn2+ show up in periplasmic space, the ions will combine with ZraS and trigger it autophosphorylation. (ZraS*)
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The phosphorylation signal will transmit from ZraS* to ZraR, then ZraR* come to ZraR(HydG) binding site[1] that will make σ54promoter start downstream transcription.
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(ZraS* ,ZraR*  means activated condition.)
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https://static.igem.org/mediawiki/parts/thumb/2/21/Lanzhou_Parts_2.png/800px-Lanzhou_Parts_2.png
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So, we substitute Zinc-Responsive Two-Component regulatory system for the copper one in BBa_K1755301. Successfully construct a new heavy metal sensor.
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Function of improved new part
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Improved new part is BBa_K2377009, which can detect the concentration of Zn2+ and transform it into electric signal.
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The downstream protein ribB (3,4-dihydroxy-2-butanone 4-phosphate synthase ,ribB) could catalyze the conversion of D-ribulose 5-phosphate formate to 3,4-dihydroxy-2-butanone 4-phosphate. This step is part of the biosynthesis pathway of riboflavin, an electron carrier in microfunction circuit (MFC).
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So when the whole gene circuit works, it will result in a consequence that once the concentration of Zn2+ in environment increased, more riboflavin will be produced to impact the voltage of MFC.
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Through this new part we realized monitoring heavy metal Zn2+ automatically, quantitatively and efficiently.
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The figure below shows the comparison of the part BBa_K1755301 (left) with the new improved part BBa_K2377009 (right), the riboflavin concentration versus heavy metal ion concentration.
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https://static.igem.org/mediawiki/parts/thumb/e/e9/Lanzhou_Parts_1.png/800px-Lanzhou_Parts_1.png
  
 
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Revision as of 17:58, 27 October 2017


zrap ribB

one element to sense zinc ion

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 625
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 515
    Illegal AgeI site found at 557
  • 1000
    COMPATIBLE WITH RFC[1000]