Difference between revisions of "Part:BBa K2305001"
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We calculated the new part’s base number to be 1007bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct. | We calculated the new part’s base number to be 1007bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct. | ||
| − | [[Image:BIT Figure Electrophoretogram K2305001-a.png|200px|left|K230501a]] [[Image:BIT Figure Electrophoretogram K2305001-b.png|200px| | + | [[Image:BIT Figure Electrophoretogram K2305001-a.png|200px|left|K230501a]] |
| + | [[Image:BIT Figure Electrophoretogram K2305001-b.png|200px|right|K230501b]] | ||
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Revision as of 17:26, 27 October 2017
cycle amplification system and GFP/RFP dual fluorescent system
Promoter plux star the line, luxl generator produce AHL, and AHL combined with luxR products will promote the expression of plux. The lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.
Agarose gel electrophoresis
We calculated the new part’s base number to be 1007bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1525
Illegal BglII site found at 2704 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4385
Illegal AgeI site found at 4497 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3383