Difference between revisions of "Part:BBa K2305002"
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promoter pbad start the line, the lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.
 
promoter pbad start the line, the lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.
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===DNA sequencing===
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The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further.
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https://static.igem.org/mediawiki/parts/a/a6/BIT_Figure_DNA_sequencing_K2305002.PNG
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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==DNA sequencing==
 
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The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 17:15, 27 October 2017


pbad promter with GFP/RFP dual fluorescent system

promoter pbad start the line, the lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.

DNA sequencing

The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further. BIT_Figure_DNA_sequencing_K2305002.PNG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1303
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2984
    Illegal AgeI site found at 3096
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1982