Difference between revisions of "Part:BBa K2275006"

 
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<partinfo>BBa_K2275006 short</partinfo>
 
<partinfo>BBa_K2275006 short</partinfo>
  
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It is used for improve function of BBa_K216005. We add a additional NsrR binding site behind B0030 which is expected to decrease the basal level of expression.
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We add an extra NsrR binding sequence at the downstream of RBS on the original part of K381001 in order to lower the basal level of expression of GFP.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:04, 27 October 2017


PyeaR(BBa_K216005) with additional NsrR binding site behind B0030 RBS

We add an extra NsrR binding sequence at the downstream of RBS on the original part of K381001 in order to lower the basal level of expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]