Difference between revisions of "Part:BBa K2382010"
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By ligating the constitutive promoter (BBa_J23101), strong ribosome | By ligating the constitutive promoter (BBa_J23101), strong ribosome | ||
binding site (BBa_B0034) and scFv-EAAAK- RFP-Histag, we were able to | binding site (BBa_B0034) and scFv-EAAAK- RFP-Histag, we were able to | ||
Revision as of 17:00, 27 October 2017
Anti-aflatoxin scFv fusion protein construction DNA sequence
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1342
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 403
Illegal SapI.rc site found at 316
Short description
By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and scFv-EAAAK- RFP-Histag, we were able to detect aflatoxin by the purified protein expressing in E. coli. Moreover, we designed a restriction site, BamHI, between scFv and EAAAK, so future iGEM teams could take advantage of this composite part to fuse their scFv with RFP as indicator, and make their own test strip!