Difference between revisions of "Part:BBa K2275005"
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<partinfo>BBa_K2275005 short</partinfo> | <partinfo>BBa_K2275005 short</partinfo> | ||
| − | We add an extra NsrR binding sequence before RBS which is expected to decrease the basal level of expression on the original part of K381001 in order to improve function of BBa_K381001 | + | We add an extra NsrR binding sequence before RBS which is expected to decrease the basal level of expression on the original part of K381001 in order to improve function of BBa_K381001. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 16:59, 27 October 2017
PyeaR(BBa_K216005) with additional NsrR binding site in front of B0030 RBS
We add an extra NsrR binding sequence before RBS which is expected to decrease the basal level of expression on the original part of K381001 in order to improve function of BBa_K381001.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 107
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]