Difference between revisions of "Part:BBa K2205002"

Revision as of 16:29, 27 October 2017

J23100-deGFP

deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants.

CFPS Expression of deGFP

CFPS Expression of J23100-deGFP and J23100-sfGFP Constructs: Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.

In vivo Qualitative Comparison

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2205002 short</partinfo>
-deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants. This is an expression part where deGFP is cloned downstream of a strong Anderson promoter (J23100) and a strong RBS (B0034). deGFP is followed by a double terminator (B0015).
+deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants.
+===CFPS Expression of deGFP===
+[[File:T--Newcastle--BB_CFPS_deGFP_sfGFP.png|500px|]]
+'''CFPS Expression of ''J23100-deGFP'' and ''J23100-sfGFP'' Constructs:''' Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.
+===''In vivo'' Qualitative Comparison===
+[[File:T--Newcastle--BB_deGFP.png|400px|]]
 <!-- Add more about the biology of this part here