Difference between revisions of "Part:BBa K2325104"

Revision as of 15:55, 27 October 2017

N20-sgRNA function device in pTarget

The BBa_K2325104 is a construct contains N20 sequence selected from the genome of T7 phage, with a constitutive promoter, which is constructed in the CRISPR/Cas system to make the E.coli be able to confer resistance to T7 phage.

Usage and Biology

Figure 1. Growth curve of BL21(DE3) (pCas+pTarget) with and without the T7 phage infection and E.coli BL21(DE3)(negative control) with the T7 phage infection in LB media.

The results convinced that the recombinant strain functioned quite well to resist the phage in LB, and the efficiency of resistance reached nearly 100%.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 41

@@ Line 5: / Line 5: @@
 The BBa_K2325104 is a construct contains N20 sequence selected from the genome of T7 phage, with a constitutive promoter, which is constructed in the CRISPR/Cas system to make the E.coli be able to confer resistance to T7 phage.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[File:BBa_K2325104--1.png]]
+<center>
+[[File:BBa_K2325104--2.png|750px]]<br />
+<b>Figure 1. Growth curve of BL21(DE3) (pCas+pTarget) with and without the T7 phage infection and <i>E.coli</i> BL21(DE3)(negative control) with the T7 phage infection in LB media.</b>
+</center>
+The results convinced that the recombinant strain functioned quite well to resist the phage in LB, and the efficiency of resistance reached nearly 100%.
 <!-- -->