Difference between revisions of "Part:BBa K2492000"
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<partinfo>BBa_K2492000 short</partinfo> | <partinfo>BBa_K2492000 short</partinfo> | ||
− | Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group we use it to | + | Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part). |
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Revision as of 15:21, 27 October 2017
GFP (optimised, for use in the C. elegans chassis)
Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 264
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 797