Difference between revisions of "Part:BBa K2407307"
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==Introduction== | ==Introduction== | ||
| − | + | It is regulated by TDH3 promoter.This composite workes for inserting the vox sites and Ura3 gene between them can be replaced by any structural gene. Initially, Before the vika-vox system works, the β-carotene does not express. After mating, the component between two vox sites will be deleted by vika enzyme and the β-carotene gene will synthesize β-carotene. Yeasts turns to orange. | |
Revision as of 15:11, 27 October 2017
TDH3 Promoter + vox + Ura + Ura3 terminator + vox + β-carotene
Introduction
It is regulated by TDH3 promoter.This composite workes for inserting the vox sites and Ura3 gene between them can be replaced by any structural gene. Initially, Before the vika-vox system works, the β-carotene does not express. After mating, the component between two vox sites will be deleted by vika enzyme and the β-carotene gene will synthesize β-carotene. Yeasts turns to orange.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2410
Illegal BglII site found at 4097
Illegal XhoI site found at 2109
Illegal XhoI site found at 2241
Illegal XhoI site found at 3060 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2277
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1390
Illegal BsaI site found at 5001
Illegal BsaI site found at 7356
Illegal BsaI.rc site found at 1837
Illegal BsaI.rc site found at 7687
Illegal SapI site found at 4401
Illegal SapI.rc site found at 1237