Difference between revisions of "Part:BBa K2428001"

Latest revision as of 13:17, 27 October 2017

Yeast-Bacteria shuttle vector

This part contains one of our other parts (BBa_K2428000, which is psB1C3mut), SCARG4 (a gene that produces arginine) and PARS2 (an autonomous replicating element). We PCRd the region containing these two genes. Using Gibson assembly, we added this part into psB1C3mut, resulting in psD000. The presence of SCARG4 and PARS2 in our construct means that psD000 is a shuttle vector (it can be transformed and expressed into both E. coli and yeast). Adding this part into any iGEM plasmid backbone will make it into a shuttle vector for Pichia pastoris and E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1602
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1767
Illegal XhoI site found at 3568
Illegal XhoI site found at 4460
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 820
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1004
Illegal SapI.rc site found at 448

Revision as of 23:42, 26 October 2017 (view source) Registry (Talk \| contribs)		Latest revision as of 13:17, 27 October 2017 (view source) Pzulueta97 (Talk \| contribs)
Line 3:		Line 3:
	<partinfo>BBa_K2428001 short</partinfo>		<partinfo>BBa_K2428001 short</partinfo>

−	This part contains one of our other parts (BBa_K2428000, which is psB1C3mut), SCARG4 (a gene that produces arginine) and PARS2 (an autonomous replicating element). We PCRd the region containing these two genes. Using Gibson assembly, we added ~~these two genes~~ into psB1C3mut, resulting in psD000. The presence of SCARG4 and PARS2 in our construct means that psD000 is a shuttle vector (it can be transformed and expressed into both E. coli and yeast).	+	This part contains one of our other parts (BBa_K2428000, which is psB1C3mut), SCARG4 (a gene that produces arginine) and PARS2 (an autonomous replicating element). We PCRd the region containing these two genes. Using Gibson assembly, we added this part into psB1C3mut, resulting in psD000. The presence of SCARG4 and PARS2 in our construct means that psD000 is a shuttle vector (it can be transformed and expressed into both E. coli and yeast). Adding this part into any iGEM plasmid backbone will make it into a shuttle vector for Pichia pastoris and E. coli.