Difference between revisions of "Part:BBa K2398000:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The sequence had to be RFC 10 compatible and therefore Biobrick prefix and suffix was added upstream and downstream of the coding sequence.
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The sequence had to be compatible with the Biobrick standard. Therefore, Biobrick prefix and suffix was added upstream and downstream of the coding sequence.
  
  

Latest revision as of 12:42, 27 October 2017


Organosilicon catalyzing Cytochrome c


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence had to be compatible with the Biobrick standard. Therefore, Biobrick prefix and suffix was added upstream and downstream of the coding sequence.


Source

The Cytochrome c is derived from the Gram-negative bacteria Rhodotermus marinus and is codon-optimized for the use in Escherichia coli. It was ordered as gBlock from IDT (Integrated DNA Technologies).

References