Difference between revisions of "Part:BBa K2440034:Design"

(Design Notes)
(Source)
 
Line 11: Line 11:
 
===Source===
 
===Source===
  
We obtain it from PCR.
+
We obtained it by annealing of two complementary single-strand oligonucleotides, which were synthesized by company as primers.
  
 
===References===
 
===References===

Latest revision as of 12:19, 27 October 2017


miR172 target sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is the reverse complementary sequence of miR172.

Source

We obtained it by annealing of two complementary single-strand oligonucleotides, which were synthesized by company as primers.

References