Difference between revisions of "Part:BBa K2440034"

 
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<partinfo>BBa_K2440034 short</partinfo>
 
<partinfo>BBa_K2440034 short</partinfo>
  
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Null.
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It is the target sequence of miR172, a modularized DNA part from a set of chemically synthetic oligo DNA library.
  
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===Usage and Biology===
 
===Usage and Biology===
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MiRNA locker assembled by using this modularized DNA part was able to bind miR172 in an Ago2 dependent manner, that is, knockdown of miR172 was achieved by transfecting cells with miRNA locker.
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===Sequence and Features===
  
 
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<partinfo>BBa_K2440034 parameters</partinfo>
 
<partinfo>BBa_K2440034 parameters</partinfo>
 
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==Experimental Validation==
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This part is sequenced as correct after construction.

Revision as of 12:18, 27 October 2017


miR172 target sequence

It is the target sequence of miR172, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR172 in an Ago2 dependent manner, that is, knockdown of miR172 was achieved by transfecting cells with miRNA locker.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.