Difference between revisions of "Part:BBa K2423000"
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<partinfo>BBa_K2423000 short</partinfo> | <partinfo>BBa_K2423000 short</partinfo> | ||
| − | This part codes for carotenoid cleavage dioxygenase 2, a member of the CCD family. This enzyme catalyses the conversion of zeaxanthin into crocetin dialdehyde in Crocus ancyrensis through cleavage at the 7,8 and 7',8' position. The part contains a constitutive promoter, and a His tag for purification. | + | This part codes for carotenoid cleavage dioxygenase 2, a member of the CCD family. This enzyme catalyses the conversion of zeaxanthin into crocetin dialdehyde in Crocus ancyrensis through cleavage at the 7,8 and 7',8' position. The part contains a constitutive promoter, and a His tag for purification. A different version of this biobrick is BBa_K2423005, which is controlled by an inducible promoter. |
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Revision as of 12:07, 27 October 2017
CaCCD2 under the control of a constitutive promoter (BBa_J23106)
This part codes for carotenoid cleavage dioxygenase 2, a member of the CCD family. This enzyme catalyses the conversion of zeaxanthin into crocetin dialdehyde in Crocus ancyrensis through cleavage at the 7,8 and 7',8' position. The part contains a constitutive promoter, and a His tag for purification. A different version of this biobrick is BBa_K2423005, which is controlled by an inducible promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1774
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 672