Difference between revisions of "Part:BBa K2440030"

 
Line 3: Line 3:
 
<partinfo>BBa_K2440030 short</partinfo>
 
<partinfo>BBa_K2440030 short</partinfo>
  
Null.
+
It is the target sequence of miR-152, a modularized DNA part from a set of chemically synthetic oligo DNA library.
  
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
MiRNA locker assembled by using this modularized DNA part was able to bind miR-152 in an Ago2 dependent manner, that is, knockdown of miR-152 was achieved by transfecting cells with miRNA locker.
 +
 +
===Sequence and Features===
  
 
<!-- -->
 
<!-- -->
Line 17: Line 20:
 
<partinfo>BBa_K2440030 parameters</partinfo>
 
<partinfo>BBa_K2440030 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
==Experimental Validation==
 +
 +
 +
This part is sequenced as correct after construction.

Revision as of 12:06, 27 October 2017


miR-152 target sequence

It is the target sequence of miR-152, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-152 in an Ago2 dependent manner, that is, knockdown of miR-152 was achieved by transfecting cells with miRNA locker.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.