Difference between revisions of "Part:BBa K2226000"

Revision as of 11:52, 27 October 2017

COX-2 promoter - FRB - Nluc398

COX-2 promoter, FRB rapamycin-binding domain (FRB) of human mTOR, fused to n-terminal of split luciferase (Nluc398), acts as one half of a promoter-reporter system, forms FRB-rapamycin-FKBP complex in prescence of rapamycin.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 45
Illegal NheI site found at 68
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 06:21, 24 October 2017 (view source) Registry (Talk \| contribs)		Revision as of 11:52, 27 October 2017 (view source) 18tsaia (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K2226000 short</partinfo>		<partinfo>BBa_K2226000 short</partinfo>

−	COX-2 promoter, ~~FK-506~~-binding ~~protein 12~~ fused to c-terminal of split luciferase (~~Cluc394~~), acts as one half of a promoter-reporter system, forms FRB-rapamycin-FKBP complex in prescence of rapamycin.	+	COX-2 promoter, FRB rapamycin-binding domain (FRB) of human mTOR, fused to n-terminal of split luciferase (Nluc398), acts as one half of a promoter-reporter system, forms FRB-rapamycin-FKBP complex in prescence of rapamycin.