Difference between revisions of "Part:BBa K2408023"

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<partinfo>BBa_K2408023 short</partinfo>
 
<partinfo>BBa_K2408023 short</partinfo>
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'''This is a part improvment from BBa_K1033121 - team Uppsala 2013'''
  
 
Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag
 
Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag

Revision as of 11:50, 27 October 2017


ADH1_Miraculin

This is a part improvment from BBa_K1033121 - team Uppsala 2013

Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag

The miraculin glycoprotein, originally produced from the Synsepalum dulcificum plant, is an effectively non-cariogenic and acceptable for diabetics in flavor and sweetening formulations and has the ability to convert the sour taste to the sweet taste, and provides a sugar substitute because it has a glycemic index of zero. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of the special protein is still not entirely clear but it is known that in the presence of food / beverage products with a pH level between 3-6 factors trigger the reaction that will give the sense of sweetness effective we added the ADH1 strong promoter at the beginning of the sequence and a histidine tag (his * 6) and terminator at the end.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 771
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1222
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1110
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93