Difference between revisions of "Part:BBa K1033121"
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'''Part improvement: team Tel-Hai 2017''' | '''Part improvement: team Tel-Hai 2017''' | ||
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BBa_K2408023 | BBa_K2408023 | ||
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Miraculin is a glycoprotein made by plant cells, therefore a yeast like <I>S. cerevisiae</I> would make a better | Miraculin is a glycoprotein made by plant cells, therefore a yeast like <I>S. cerevisiae</I> would make a better | ||
expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: 1. ADH1 - strong constitutive promoter for <I>S. cerevisiae</I>. 2. His tag for purification 3. ADH1 terminator for more effective expression. | expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: 1. ADH1 - strong constitutive promoter for <I>S. cerevisiae</I>. 2. His tag for purification 3. ADH1 terminator for more effective expression. | ||
Revision as of 11:49, 27 October 2017
Miraculin
Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
Applications
Miraculin can be used as a sweetener.
Part improvement: team Tel-Hai 2017
BBa_K2408023
Miraculin is a glycoprotein made by plant cells, therefore a yeast like S. cerevisiae would make a better
expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: 1. ADH1 - strong constitutive promoter for S. cerevisiae. 2. His tag for purification 3. ADH1 terminator for more effective expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]