Difference between revisions of "Part:BBa K2492002:Experience"
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Since we need to express protein in C.elegans, introns should be added to achieve a good expression level of protein. The part BBa_K2005050 is reporter mcherry(a kind of red fluorescence protein), we get the sequence of mcherry and add introns.
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Universal mCherry coding sequence of three introns for good expression in C.elegans.
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The mcherry part we use is optimized for C.elegans and perform excellently under confocal microscope. The whole neuron with dendrite can be observed distinctly. We can even observe the branch of dendrite near the mouse.
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MCherry is a commonly used reporter. Since ordinary mCherry(part BBa_K2005050) may not have high expression in C.elegans, artificial introns are inserted in the constructed plasmid[1]. We can obtain ord-10::Chrimson::GEM-GECO::mCherry transgenic offsprings microinjection. MCherry driven by promoter ord-10(Part:BBa_K2492004) indicates the position of neuron AWA. Moreover the fluorescence of mCherry aids the imaging for the neuron AWA pattern. The intensity of fluorescence reveals the expression level approximately.
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The mCherry part we used now is optimized for C.elegans and perform excellently observed under confocal microscope. We added the figure to part page The whole neuron with dendrite can be observed distinctly. The branch of dendrite near the mouse can be observed as well(Figure).
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[1]:S.Redemann. (2011). codon adaptation– based control of protein expression in C. elegans, Nature Methods, Vol.8 No.3 250-254.
  
 
===Applications of BBa_K2492002===
 
===Applications of BBa_K2492002===

Revision as of 11:36, 27 October 2017


Universal mCherry coding sequence of three introns for good expression in C.elegans. MCherry is a commonly used reporter. Since ordinary mCherry(part BBa_K2005050) may not have high expression in C.elegans, artificial introns are inserted in the constructed plasmid[1]. We can obtain ord-10::Chrimson::GEM-GECO::mCherry transgenic offsprings microinjection. MCherry driven by promoter ord-10(Part:BBa_K2492004) indicates the position of neuron AWA. Moreover the fluorescence of mCherry aids the imaging for the neuron AWA pattern. The intensity of fluorescence reveals the expression level approximately. The mCherry part we used now is optimized for C.elegans and perform excellently observed under confocal microscope. We added the figure to part page The whole neuron with dendrite can be observed distinctly. The branch of dendrite near the mouse can be observed as well(Figure).

[1]:S.Redemann. (2011). codon adaptation– based control of protein expression in C. elegans, Nature Methods, Vol.8 No.3 250-254.

Applications of BBa_K2492002

mcherry expressed in AWA

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