Difference between revisions of "Part:BBa K2492002"
Line 5: Line 5:
 
Mcherry, RFP, with three introns added. Mcherry is following the promoter ord-10, which can lead to high expression in AWA. We use this modified mcherry to locate the neuron AWA in C.elegans.  
 
Mcherry, RFP, with three introns added. Mcherry is following the promoter ord-10, which can lead to high expression in AWA. We use this modified mcherry to locate the neuron AWA in C.elegans.  
  
 +
[[File: T--SUSTech_Shenzhen--C.elegans_AWA_mcherry.png|500px|thumb|left|mcherry expressed in AWA]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
−
[[File: T--SUSTech_Shenzhen--C.elegans_AWA_mcherry.png|500px|thumb|left|mcherry expressed in AWA]]
 
  
 
<!-- -->
 
<!-- -->

Revision as of 11:26, 27 October 2017


MCherry optimized with three introns for C.elegans

Mcherry, RFP, with three introns added. Mcherry is following the promoter ord-10, which can lead to high expression in AWA. We use this modified mcherry to locate the neuron AWA in C.elegans.

mcherry expressed in AWA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3