Difference between revisions of "Part:BBa K116404:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| − | how you used this part and how it worked out. | + | how you used this part and how it worked out. |
| + | By replacing the weaker RBS BBa_B0032 of the original part with a stronger RBS BBa_B0034, we have successfully constructed an improved phosphate sensor-GFP reporter BBa_K2447000 (https://parts.igem.org/Part:BBa_K2447000). Our part shows, on average, 40 fold increase in GFP expression when compared to the previous version of the construct. The original phosphate construct is also insensitive to high phosphate concentrations above 50 µM where similar levels of GFP expression are observed (Figure 4). Unlike the previous construct, our improved phosphate construct is much more sensitive to various phosphate concentrations from 0 to 1000 µM, particularly at phosphate concentrations above 50 µM. | ||
===Applications of BBa_K116404=== | ===Applications of BBa_K116404=== | ||
Revision as of 10:49, 27 October 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
By replacing the weaker RBS BBa_B0032 of the original part with a stronger RBS BBa_B0034, we have successfully constructed an improved phosphate sensor-GFP reporter BBa_K2447000 (https://parts.igem.org/Part:BBa_K2447000). Our part shows, on average, 40 fold increase in GFP expression when compared to the previous version of the construct. The original phosphate construct is also insensitive to high phosphate concentrations above 50 µM where similar levels of GFP expression are observed (Figure 4). Unlike the previous construct, our improved phosphate construct is much more sensitive to various phosphate concentrations from 0 to 1000 µM, particularly at phosphate concentrations above 50 µM.
Applications of BBa_K116404
User Reviews
UNIQd8423f851a5bdf3e-partinfo-00000000-QINU UNIQd8423f851a5bdf3e-partinfo-00000001-QINU