Difference between revisions of "Part:BBa K2423002"
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<partinfo>BBa_K2423002 short</partinfo> | <partinfo>BBa_K2423002 short</partinfo> | ||
| − | This part codes for a cytosolic glycosyltransferase found in Crocus sativus. The enzyme will catalyse the conversion of crocetin to α-crocin in the presence of UDP-glucose. It is a modified version of BBa_K1033112, with two stop codons and a SGGS linker for the His tag. | + | This part codes for a cytosolic glycosyltransferase found in Crocus sativus. The enzyme will catalyse the conversion of crocetin to α-crocin in the presence of UDP-glucose. It is a modified version of BBa_K1033112, with two stop codons and a SGGS linker for the His tag. BBa_K2423008 is a slightly modifed version of this part (it uses BBa_J04500 as a promoter and RBS instead). |
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Revision as of 10:26, 27 October 2017
UGTCs2 under the control of a constitutive promoter (BBa_J23106)
This part codes for a cytosolic glycosyltransferase found in Crocus sativus. The enzyme will catalyse the conversion of crocetin to α-crocin in the presence of UDP-glucose. It is a modified version of BBa_K1033112, with two stop codons and a SGGS linker for the His tag. BBa_K2423008 is a slightly modifed version of this part (it uses BBa_J04500 as a promoter and RBS instead).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 593
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 766
Illegal AgeI site found at 1121 - 1000COMPATIBLE WITH RFC[1000]