Difference between revisions of "Part:BBa K2423008"
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The gene of interest in this part is regulated by BBa_J04500. This implies that it has to be induced by lactose or any of its closely related derivates, such as IPTG. | The gene of interest in this part is regulated by BBa_J04500. This implies that it has to be induced by lactose or any of its closely related derivates, such as IPTG. | ||
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| + | This BioBrick is an improved version of BBa_K1033112 and a slightly modified version of BBa_K2423002 (different promoter and RBS). | ||
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Revision as of 10:24, 27 October 2017
UGTCs2 with BBa_J04500
UGTCs2 is an enzyme that catalyzes the conversion of crocetin to crocin. This is the third and final step in the zeaxanthin to crocin pathway. In nature this enzyme can be found in Crocus Sativus. Crocus Sativus is the plant where saffron is harvested from.
The gene of interest in this part is regulated by BBa_J04500. This implies that it has to be induced by lactose or any of its closely related derivates, such as IPTG.
This BioBrick is an improved version of BBa_K1033112 and a slightly modified version of BBa_K2423002 (different promoter and RBS).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 758
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 931
Illegal AgeI site found at 1286 - 1000COMPATIBLE WITH RFC[1000]